Table 2.

Primers used for quantification of cytokine mRNA expression by qPCR.

Target	Oligo	Oligonucleotide sequence (5′ → 3′)	Product size (bp)	References
IL-2	Frw	GCATCGCACTGACGCTTGTA	86	(34)
	Rev	TTGCTCCATCTGTTGCTCTGTT
IL-4	Frw	CATCCTCACAGCGAGAAACG	83	(35)
	Rev	CCTTATCGCTTGTGTTCTTTGGA
IL-5	Frw	GCCTATGTTTCTGCCTTTGC	106	(36)
	Rev	GGTTCCCATCGCCTATCA
IL-10	Frw	CAAGCCCTGTCGGAGATGAT	78	(37)
	Rev	CTTGATGTCTGGGTCGTGGTT
IL-12p40	Frw	CAGCAGAGAGGGTCAGAGTGG	109	(34)
	Rev	ACGACCTCGATGGGTAGGC
TNF-α	Frw	AATCATCTTCTCGAACCCCAAGT	74	(38)
	Rev	GGAGCTGCCCCTCAGCTT
TGF-β	Frw	CAGAATGGCTGTCCTTTGATGTC	79	(35)
	Rev	AGGCGAAAGCCCTCGACTT
IFN-γ	Frw	TCAACCCCTTCTCGCCACT	113	(36)
	Rev	GCTGCCTACTTGGTCCCTGA
β-actin	Frw	ACGGAGCGTGGCTACAGC	62	(38)
	Rev	TCCTTGATGTCACGCACGA

bp, base pair; Frw, forward primer; Rev, reverse primer.