Table 3.
Parameterization and objective function value obtained by fitting different models for the production of A/PR/8/34-delS1(1) by MDCK.SUS2 cells in a parallel continuous bioreactor system at residence time 36 h.
| Parameters | Model 1, all parameters | Model 2, kdvit = kvdt = 0 | Model 3, kvi = kvidi | Model 4, kdvit = kvdt = 0 and kvi = kvidi |
|---|---|---|---|---|
| kvi | 5.38·10−8 | 1.38·10−7 | 2.89·10−9 | 2.60·10−9 |
| kvidi | 7.96·10−11 | 1.16·10−10 | kvi | kvi |
| kcdv | 0.003 | 0.002 | 0.001 | 0.001 |
| μvi | 4.12 | 1.42 | 142 | 80 |
| μvddiC | 177 | 93 | 152 | 153 |
| μvddiS | 1.13·10−9 | 2.57·10−7 | 1.04·10−10 | 1.62·10−7 |
| μvdC | 1.41·10−8 | 1.42·10−9 | 1.01·10−5 | 2.01·10−6 |
| μvdS | 173 | 193 | 613 | 725 |
| kdvit | 0.07 | 0 | 4.35·10−3 | 0 |
| kvdt | 2.02·10−9 | 0 | 2.15·10−8 | 0 |
| Objective funciton value# | 6.19 | 13.36 | 4.99 | 5.06 |
#
Objective function values are the normalized least squared prediction errors of the state variables of all cells Cellstotal, fully infectious STVs VS, replication-incompetent DIPs Vddi, and non-infectious FL S1-containing virions Vd.