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. 2019 Oct 18;12:257. doi: 10.3389/fnmol.2019.00257

FIGURE 2.

FIGURE 2

[3H]CP55940 and [35S]GTPγS binding to hCB1R. (A) [3H]CP55940 (0.7 nM) binding to membranes obtained from CHO cells transfected with hCB1R was measured in the presence of indomethacin. Symbols represent mean percentage changes in [3H]CP55940 binding values ± SEM. Asterisks indicate mean values that are significantly different from zero via Student’s one sample t-test (∗∗P < 0.01, ∗∗∗P < 0.001). Data are presented as mean values ± SEM. N = 4–6. (B) Effect of 1 μM indomethacin on the kinetics of [3H]CP55940 for its dissociation from binding sites on membranes obtained from hCB1R CHO cells. Data were best fitted using a one-phase dissociation model. Data are presented as mean values ± SEM. N = 5. (C) The effects of indomethacin on [35S]GTPγS binding in CHO cells expressing hCB1R treated with AEA in the presence of DMSO or 1 μM indomethacin. Asterisks indicate mean values that are significantly different from zero via Student’s one sample t-test (∗P < 0.05). Data are mean ± SEM. N = 5–6.