Skip to main content
. 2019 Oct 18;10:1189. doi: 10.3389/fphar.2019.01189

Figure 1.

Figure 1

Effects of MP on ConA−induced EAH. (A) Animal study protocol. (B) Serum ALT and AST. (C) Pathological liver specimens stained with H&E (×200): The control group showed normal hepatic architecture, while ConA injection induced marked pathological damage, including dramatic inflammatory cell infiltration in the portal area, massive cloudy swelling, blood vessel congestion and dilatation, and disordered hepatic sinusoid structures. MP-treated animals showed improvements of these lesions. (D) Serum IL-6 and IFN-γ. (E) Immunohistochemistry was used to show CD4+ T cell infiltration (original magnification, ×200). ConA injection in the EAH group induced a significant increase in the number of infiltrating CD4+ T cells compared to that in the control group. The percentage of CD4+ T cells was significantly decreased after MP treatment and almost returned to normal levels compared to that in the EAH group. Data are presented as the mean ± SD (n = 8). **P < 0.01 vs. control group; #P < 0.05, ##P < 0.01 vs. EAH group. ConA, concanavalin A; MP, methylprednisolone; NC, normal control; EAH, experimental autoimmune hepatitis; ALT, alanine aminotransferase; AST, aspartate aminotransferase; IL-6, interleukin-6; IFN-γ, interferon-γ; IOD, integrated optical density.