TABLE 1.
Oligonucleotide primers used for the amplification of genus, species and virulence genes of C. jejuni isolates.
Target gene | Primer name | Oligonucleotide sequence (5′→3′) | Amplicon size (bp) | Reference |
23S rRNA | 23SF | TATACCGGTAAGGAGTGCTGGAG | 650 | Wang et al., 2002 |
23SR | ATCAATTAACCTTCGAGCACCG | |||
hipO | CJF | ACTTCTTTATTGCTTGCTGC | 323 | Wang et al., 2002 |
CJR | GCCACAACAAGTAAAGAAGC | |||
cdtA | GNW | GGAAATTGGATTTGGGGCTATACT | 165 | Wieczorek et al., 2012 |
IVH | ATCACAAGGATAATGGACAAT | |||
cdtB | VAT2 | GTTAAAATCCCCTGCTATCAACCA | 495 | Wieczorek et al., 2012 |
WMI-R | GTTGGCACTTGGAATTTGCAAGGC | |||
cdtC | WMI-F | TGGATGATAGCAGGGGATTTTAAC | 555 | Wieczorek et al., 2012 |
LPF-X | TTGCACATAACCAAAAGGAAG | |||
flaA | FLA242FU | CTATGGATGAGCAATT(AT)AAAAT | 402 | Meinersmann et al., 1997 |
FLA625RU | CAAG(AT)CCTGTTCC(AT)ACTGAAG | |||
virB11 | virB-232 | TCTTGTGAGTTGCCTTACCCCTTTT | 494 | Datta et al., 2003 |
virB-701 | CCTGCGTGTCCTGTGTTATTTACCC | |||
wlaN | wlaN DL-39 | TTAAGAGCAAGATATGAAGGTG | 672 | Kordinas et al., 2005 |
wlaN DL-41 | CCATTTGAATTGATATTTTTG | |||
iam | IAMF | GCGCAAATATTATCACCC | 518 | Wieczorek et al., 2012 |
IAMR | TTCACGACTACTACTATGCGG |