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. 2019 Oct 18;10:1308. doi: 10.3389/fphys.2019.01308

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Copyright © 2019 Lin, Ma, Kuo, Su, Wang, Chan, Su, Weng, Yang, Lin and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Elf3 knockdown reduced basal and vasopressin-induced AQP2 gene expression in the mpkCCD cells. (A) Elf3 and (B) AQP2 mRNA levels in the control (shCon) or stable Elf3 knockdown cells in response to vehicle (Veh) or 0.1 nM dDAVP (AVP) stimulation for 8 h. Values are means ± SE, adjusted for loading (RPLP0 mRNA levels) and normalized against the values of the control cells under the vehicle conditions. Asterisk indicates significant difference (^∗p < 0.05; ^∗∗p < 0.01; and ^∗∗∗p < 0.001, t-test). (C) AQP2 mRNA ratios summarized from the experiments shown in (B). (D) Transepithelial resistance of the control and stable Elf3 knockdown cells grown on Transwell prior to the experiments. (E,F) Representative and summary of immunoblotting for AQP2 protein in the control and stable Elf3 knockdown cells in response to vehicle or dDAVP. Values are means ± SE, adjusted for loading (β-actin staining) and normalized against values in the control cells under the vehicle conditions. Cell lysate from hamster inner medullary collecting duct (IMCD) serves as a positive control. β-actin serves as a loading control.