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. 2019 Oct 18;10:1308. doi: 10.3389/fphys.2019.01308

FIGURE 4.

FIGURE 4

Vasopressin did not induce Elf3 nuclear translocation in the mpkCCD cells. (A) confocal immunofluorescence micrographs of mpkCCD cells transfected with the V5-tagged Elf3 isoform 1 expression vector and stimulated with dDAVP for 30 min. (B) Nucleus (Nc) vs. cytosol (Cyt) fractionation followed by immunoblotting for V5-tagged Elf3 in the mpkCCD cells under vehicle or dDAVP-stimulated conditions. GAPDH and histone H2A were stained as a cytosol and a nucleus marker, respectively. In, input i.e., cell lysate.