FIGURE 2.

Diurnal sleep–wake cycles were affected by WIRS. (A) Experimental timeline and example of EEG (above) and EMG (bottom) patterns in each stage of wake (left), NREMS (middle), and REMS (right). (B–D) Daily variation in wake (B), NREMS (C), and REMS (D) every 3 h. Because the baseline recording was only 24 h, the same data were used as the control for the first and second day recordings at 1W, 2W, and 3W. N = 7. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001, two-way repeated measures ANOVA followed by Bonferroni’s multiple comparisons test. Two-way repeated measures ANOVA was applied over the entire 45 h recording period. Data are presented as means ± SEM.