Fig. 3.

Light- and temperature-inducible recombinases. a A schematic showing a split Cre fused to nMag and pMag blue light-inducible dimerization domains. b Pulses indicating the changing light (left) and temperature (center and right) conditions. Plots indicate GFP mean fluorescence intensity (MFI) of various nMag/pMag-tagged split Cre (c) and Flp (d) recombinases under blue light (blue squares), room temperature (pink squares), and refrigeration (purple squares). Black squares indicate samples treated with constant temperature and dark conditions. PA-Cre indicates expression of a previously developed blue light-inducible Cre recombinase³. Inducible recombinases were compared with samples transfected with constitutive recombinases or a blank vector. Error bars of MFI indicate the arithmetic standard error of the mean between three transfected cell cultures. Source data are provided as a Source Data file