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. 2019 Oct 24;51(10):124. doi: 10.1038/s12276-019-0320-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b qPCR for detecting the expression of genes, including pu.1, csf1ra, mpx, il6r, il6, and gp130, from purified HSC-independent macrophages (mpeg1:GFP⁺) and neutrophils (mpx:GFP⁺) at 30 hpf. Expression was normalized to ef1a and is presented relative to whole-embryo expression. Bars represent the means ± SEM of duplicate samples. *P < 0.05; **p < 0.01; ***p < 0.001; n.s., not significant. c Std and Pu.1 morphants were examined by WISH for runx1 expression at 28 hpf. d Qualitative phenotype distribution of embryos from c scored with medium, high, and low runx1 expression. Medium, white bar; high, red bar; low, gray bar. e Std, Irf8, and Il6 + Irf8 morphants were examined by WISH for runx1 and mpx at 29 hpf. Black arrowheads denote runx1⁺ HSCs in the DA region and mpx⁺ neutrophils in the PBI and trunk regions. f Qualitative phenotype distribution of embryos from e scored as in d. g Whole-embryo expression of il6 and gata2b relative to ef1a in Std, Pu.1, and Irf8 morphants at 28 hpf. Bars represent the means ± SEM of duplicate samples. **P < 0.01; ***p < 0.001