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. 2019 Oct 24;10(11):806. doi: 10.1038/s41419-019-2002-6

Fig. 11. Effects of LLOMe on autophagy and cellular injury after exposure of podocytes to AGE-BSA.

Fig. 11

a, c Immunofluorescence staining of LC3 or p62 in podocytes after exposure to LLOMe. b, d Quantification of LC3 or p62 expression after exposure of podocytes to LLOMe. LLOMe induced an accumulation of LC3 or p62 in podocytes. e Fluorescence microscopic analysis of podocytes transfected with tfLC3 and treated with LLOMe. f Quantitative data for green or red puncta per cell. g Quantitative data for yellow puncta or free red puncta per cell. Autophagosomes (arrowheads), but not autolysosomes (arrows) were increased after exposure to LLOMe. h Immunofluorescence of F-actin cytoskeleton (green) showed loss of stress fibers and cortical actin staining after exposure of podocytes to LLOMe. i Quantification of the phalloidin staining. j The activity of caspase-3 was increased after exposure of podocytes to LLOMe. Scale bar, 10 μm