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. 2019 Oct 24;7(20):e14272. doi: 10.14814/phy2.14272

Figure 2.

Figure 2

TRPM7‐like currents in differentiated 3T3‐L1 adipocytes. (A) The expression of TRPM7 mRNA in native white adipocytes and 3T3‐L1 adipocytes. Each bar represents the mean ± SEM (vertical bar) of 4 to 5 observations. (B) A representative trace of whole‐cell currents showing the time course of current activation by an application of divalent‐free solution (DVF) and inhibition by the addition of H2O2 (500 μmol/L) in a differentiated 3T3‐L1 adipocyte in the presence of 29 μmol/L [Mg2+]i. The current amplitude at +80 mV (closed circles) or −80 mV (open circles) was plotted against the recording time. (C) Representative I–V relationship of TRPM7‐like current recorded from the same cell shown in B under the control (black line), DVF (red line), or 4 min after application of H2O2 (500 μmol/L) (blue line). (D) DVF induced a substantial increase in both inward and outward currents in 3T3‐L1 adipocytes. Each bar represents the mean ± SEM (vertical bar) of 9 observations. *P < 0.05 versus DVF. (E–G) Effect of 2‐APB (200 μmol/L) (E), NMM (100 μmol/L) (F), and NS8593 (20 μmol/L) (G) on the DVF‐activated, TRPM7‐like current in 3T3‐L1 adipocytes. Each bar represents the mean ± SEM (vertical bar) of 6 to 8 observations. *P < 0.05 versus DVF.