Fig. 1.

CC-seq maps covalent Spo11-linked DNA breaks in S. cerevisiae meiosis with nucleotide accuracy. a Schematic of the CC-seq method. b Silica-based enrichment of meiotic S. cerevisiae Spo11-linked DNA fragments detected by Southern blotting at the his4::LEU2 recombination hotspot. Arrowheads indicate expected sizes of Spo11-DSBs. ‘+PK’ = samples treated with proteinase K prior to electrophoresis. The eluate was loaded at 4X cell equivalents relative to the input. c Nucleotide resolution mapping of meiotic S. cerevisiae Spo11 hotspots by CC-seq or oligo-seq²⁸. Red and blue traces indicate Spo11-linked 5′ DNA termini on the Watson and Crick strands, respectively. Grey arrows indicate positions of gene open reading frames. d Pearson correlation (r) of Spo11 CC-seq signal between Watson and Crick strands, offset by the indicated distances. Cartoon shows the known Spo11-DSB structure. HpM Hits per million mapped reads per base pair. Source data are provided as a Source Data file