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. 2019 Oct 24;2:389. doi: 10.1038/s42003-019-0624-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Loss of ATF3 aggravated the expression of inflammation-related genes in HFD-induced obese mice. a ATF3 protein level in iWAT and BAT of wild-type and ATF3^−/− mice after HFD feeding for 12 weeks. b Representative immunofluorescence images of adiponectin (red IF) and ICAM-1 (green IF) in wild-type and ATF3^−/− mice. Yellow scale bar indicated the size of adipocyte tissues. c Serum protein levels of adipokine and inflammation-related genes in wild-type and ATF3^−/− mice after HFD feeding for 8 weeks by adipokine assays; Gel-Pro Analyzer software was used for densitometry of blots. d Serum protein level of adiponectin, ICAM-1 and resistin by ELISA assays in wild-type and ATF3^−/−mice after HFD feeding for 8 weeks. e Quantified real-time PCR analysis of mRNA levels of iNOS, IL-6, and TNFα in livers of wild-type and ATF3^−/− mice. For a–c, n = 3 per group. For d, wild-type (n = 4 in adiponectin; n = 5 in ICAM1; n = 8 in resistin), ATF3^−/− (n = 7 in adiponectin; n = 5 in ICAM1; n = 7 in resistin). For e, n = 6 per group. Scale bar for image b: 50 µm. Data are mean ± SEM; *p < 0.05 compared to wild-type