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. 2019 Oct 24;10(11):809. doi: 10.1038/s41419-019-2035-x

Fig. 4. Chaetocin-induced accumulation of ROS is crucial for GC cell apoptosis.

Fig. 4

a ROS levels were analyzed by flow cytometry in HGC-27 and AGS cells treated for 1 h with 5 mM NAC, chaetocin (200 nM for HGC-27, 100 nM for AGS), or both. Results were shown as mean ± SD of three independent experiments. ***P < 0.001. HGC-27 and AGS cells were pretreated with 5 mM NAC for 1 h and then cotreated with chaetocin (200 nM for HGC-27, 100 nM for AGS). b Apoptosis was measured by flow cytometry. Results were shown as mean ± SD of three independent experiments. ***P < 0.001. c Expression levels of cleaved-PARP, caspase-3, caspase-9 and caspase-8 were analyzed by western blot. Blots presented here are representative of three independent experiments