TABLE 2.

Recent researches of CRISPR pooled library screening in pancreatic cancer.

Hit genes	Effect	Cell line	Library	Gene/sgRNA number	Selection condition	References
PSMA6	apoptosis↑, spheroid formation↓	PANC-1	Human sgRNA library Brunello (addgene No. 73178)	19114/76441	100 nM gemcitabine for 6 days	Bakke et al., 2019
Wnt pathway genes, FZD5	Proliferation↓	HPAF-II, AsPC-1, PaTu8988S	Toronto KnockOut (TKO) CRISPR Library (addgene No. 1000000069)	17232/91320	different time point (Day 15, 27, 31, 35)	Steinhart et al., 2017
CIC, ATXN1L	sensitivity to trametinib↓	PATU8902	GeCKOv2 library (addgene No. 1000000049)	19050/123411	100 nM trametinib for 14 days	Wang et al., 2017
		PATU8988T	Avana library Doench et al., 2016	18675/110257	10 nM trametinib for 14 days
CENPE, RRM1	sensitivity to trametinib↑	PDX366	Nuclear proteins gRNA sub-pool library (addgene No. 51047)	7114/73151	MEK and CENPE inhibitor treatment in 6–8-week-old mail athymic nude mice	Szlachta et al., 2018
DCK, DCTPP1	sensitivity to NUC-1031, gemcitabine↓	Mia PaCa-2, A2780	GeCKOv2 library (addgene No. 1000000049)	19050/123411	15 nM gemcitabine or 65 nM NUC-1031 for 14 days or 21 days	Sarr et al., 2019

↑, up-regulation; and ↓, down-regulation.