Table 4.
Sperimental data obtained from bovine and buffalo milk on contaminated field samples
| Kit | Matrix | Average yield | LOD IS900 target copy/μl | LOD UFC/ml |
|---|---|---|---|---|
| Nucleo Spin Food kit (Macherey Nagel) | Bovine colostrum | 22.45 ng/μl | 4 × 104−4.08 | 2.6 × 106−26.7 |
| Nucleo Spin Food kit (Macherey Nagel) | buffalo colostrum | 16.4 ng/μl | 4.28 × 105−3.91 | 2.8 × 106−26.6 |
| Nucleo Spin Food kit (Macherey Nagel) combined with magnetic beads | Bovine colostrum | 22.45 ng/μl | 4.11 × 105−4.13 | 1.33 × 106−13.8 |
| Nucleo Spin Food kit (Macherey Nagel) combined with magnetic beads | buffalo colostrum | 10 ng/μl | 4.11 × 105−4.13 | 1.33 × 105−13.8 |
| Qiamp Cador Pathogen (QIAGEN) | Bovine colostrum | 10 ng/μl | 4 × 103−10 | 4.1 × 106−1.2 × 104 |
| Qiamp Cador Pathogen (QIAGEN) | buffalo colostrum | 10 ng/μl | 5.3 × 105−53 | 4.1 × 106−4.1 × 103 |