Figure A1.

Binding and phagocytosis of Vibrio fischeri and Vibrio harveyi to hemocytes isolated from aposymbiotic (apo)‐raised, symbiotic‐raised, and wild‐caught adult animals; general hemocyte behavior. (a) The average number of bacteria bound per hemocyte. ^†Levels of bacterial binding that were significantly different from each other within an animal treatment, p < 0.0001. *Levels of V. fischeri binding that were significantly different apo‐raised animals but not each other p < 0.001. ^#Levels of V. harveyi binding that are significantly different from wild‐caught animals but not each other, p < 0.0001. Statistical significance was determined by two‐way ANOVA with post hoc pairwise comparisons using the Sidak's correction for multiple comparisons. (b) The average number of bacteria phagocytosed per hemocyte. ^†Levels of bacterial internalization that were significantly different from each other within an animal treatment, p < 0.0001. *Levels of V. fischeri internalization that were significantly different from wild‐caught animals but not each other, p < 0.0001. ^#Levels of V. harveyi binding that were significantly different from each other p < 0.01. Statistical significance was determined by two‐way ANOVA with post hoc pairwise comparisons using the Sidak's correction for multiple comparisons. (a, b) Bars represent the average ± SE. N is the total number of hemocytes used for analysis representing 5–7 biological replicates with a minimum of 30 hemocytes chosen at random per replicate for analysis