Table A2.

Abundance of OTUs analyzed (>0.00025%) in Costa Rican hot springs, closely related sequence in GenBank database and growth temperature limits

OTU	Accession N°	% of Total Reads	Closely related sequence (Accession N°)a	Similarity %	Phylum	Abundance									Growth Temp. (°C)
						MV1b	MV2	RN2	RN3	BP1	BP2	RC1	RC2	RC3
OTU143	MK040660	0.87	Chloracidobacterium thermophilum B (CP002514)	98	Acidobacteria	98	2	0	0	0	0	0	1803	533	42–60
OTU1483	MK040665	0.03	Stenotrophobacter roseus strain Ac_15_C4 (NR146022)	99	Acidobacteria	4	0	71	0	6	2	0	0	0	37–55
OTU1423	MK040659	0.05	Uncultured Acidobacteria bacterium clone YNP_SBC_BP4_B26 (HM448257)	98	Acidobacteria	0	0	0	0	0	0	140	0	0	63
OTU1630	MK077654	0.03	Uncultured bacterium clone: OK06 (AB559014)	99	Acidobacteria	38	2	17	11	0	1	0	0	2	37–60
OTU1484	MK040623	0.03	Uncultured bacterium clone BJGMM−3s−108 (JQ800904)	97	Armatimonadetes	17	0	0	39	0	0	0	3	11	59–60
OTU550	MK040716	0.1	Uncultured bacterium clone HV‐16 (GU233849)	99	Armatimonadetes	0	0	1	123	0	0	157	10	0	55–63
OTU3506	MK040695	0.18	Uncultured bacterium clone Tat‐08‐003_12_23 ( GU437312)	97	Armatimonadetes	8	0	3	22	0	0	133	255	76	55–63
OTU1741	MK040672	0.04	Dyadobacter ginsengisoli strain: Gsoil 043(T) (AB245369)	85	Bacteroidetes	0	1	0	0	106	0	0	0	2	42–60
OTU2588	MK040681	0.03	Flexibacter ruber ATCC 23,103 (M58788)	89	Bacteroidetes	0	0	0	0	0	97	0	0	0	37
OTU2087	MK077656	0.08	Saprospira grandis (AB088636)	84	Bacteroidetes	0	0	0	0	0	223	0	0	0	37
OTU67	MK040717	0.09	Uncultured bacterium clone P060905_H09 (HQ385626)	97	Bacteroidetes	0	0	229	7	0	2	0	0	0	37–59
OTU447	MK040712	0.04	Uncultured bacterium gene for 16S rRNA, partial sequence, clone: BC10‐8 (AB580674)	94	Bacteroidetes	0	123	0	0	0	0	0	0	0	42
OTU2031	MK040674	0.03	Uncultured Bacteroidetes bacterium clone Uvmin2_8 (KJ611546)	99	Bacteroidetes	0	78	2	0	0	0	0	0	0	42–55
OTU3175	MK040688	0.04	Uncultured Bacteroidetes bacterium clone YNP_SBC_FC_B31 (HM448393)	96	Bacteroidetes	0	0	0	0	0	0	98	3	0	59–63
OTU90	MK040722	0.49	Uncultured Bacteroidetes bacterium clone YNP_SBC_MS3_B18 (HM448177)	91	Bacteroidetes	0	0	0	10	0	0	301	400	656	59–63
OTU29	MK077660	0.07	Uncultured Bacteroidetes bacterium clone YNP_SBC_MS3_B22 (HM448178)	94	Bacteroidetes	9	155	18	5	7	0	0	0	8	42–60
OTU59	MK077666	0.16	Uncultured Flavobacteriales bacterium clone ED5‐012 (FJ764420)	88	Bacteroidetes	0	0	0	0	0	0	286	133	18	59–63
OTU932	MK040724	0.03	Uncultured Sphingobacteriales bacterium clone L2‐2 (JF703526)	92	Bacteroidetes	0	68	1	0	1	2	0	0	0	37–55
OTU34	MK040637	0.03	Uncultured Sphingobacteriales bacterium clone ST31 (JQ723651)	95	Bacteroidetes	0	0	2	0	0	81	0	0	0	37–55
OTU175	MK077655	0.09	Uncultured Sphingobacterium sp. clone QLBB088 (AY862023)	85	Bacteroidetes	251	4	0	0	0	0	0	0	1	42–60
OTU4172	MK040709	0.15	Ignavibacterium album(T) (CP003418)	97	Chlorobi	158	0	22	77	0	0	0	102	60	55–60
OTU27	MK077659	0.07	Uncultured bacterium clone: HAuD‐LB4(AB113613)	86	Chlorobi	51	0	17	120	0	0	0	0	0	55–59
OTU3864	MK040701	0.03	Uncultured Bacteroidetes/Chlorobi group bacterium clone SM1A03 (AF445646)	89	Chlorobi	0	70	0	1	0	0	0	0	0	42–59
OTU2226	MK040677	0.05	Uncultured Chlorobi bacterium clone Aug‐VN130 (JQ795339)	95	Chlorobi	0	0	0	0	0	0	0	63	80	59–60
OTU913	MK077669	0.05	Uncultured Chlorobi bacterium clone SM2A03 (AF445706)	98	Chlorobi	51	0	7	10	0	0	0	5	62	55–60
OTU102	MK040646	0.24	Uncultured sludge bacterium A12b (AF234699)	86	Chlorobi	6	0	1	18	0	0	442	156	51	55–63
OTU3668	MK040697	0.03	Caldilinea aerophila DSM 14535(T) (AP012337)	97	Chloroflexi	0	0	0	17	0	0	50	20	0	59–63
OTU19	MK040635	0.17	Chloroflexi bacterium Um‐2 (KP341999)	93	Chloroflexi	8	2	442	14	0	0	0	0	0	42–59
OTU119	MK040632	4.76	Chloroflexus aurantiacus J‐10‐fl(T) (D38365)	97	Chloroflexi	1818	0	20	7	2	0	6,532	2,142	2,731	55–63
OTU1488	MK040666	0.81	Chloroflexus aurantiacus J‐10‐fl(T) (D38365)	99	Chloroflexi	653	1	954	640	0	0	0	0	0	42–59
OTU40	MK040707	7.13	Chloroflexus aurantiacus J‐10‐fl(T) (D38365)	97	Chloroflexi	3,809	2	1,299	908	2	0	8,252	2,589	3,010	42–63
OTU472	MK040714	0.09	Chloroflexus aurantiacus J‐10‐fl(T) (D38365)	97	Chloroflexi	175	0	0	1	0	0	40	13	24	59–63
OTU82	MK040641	0.07	Chloroflexus aurantiacus J‐10‐fl(T) (D38365)	95	Chloroflexi	200	0	2	1	0	0	0	1	0	55–59
OTU116	MK040630	1.29	Roseiflexus castenholzii(T) DSM 13941 (CP000804)	95	Chloroflexi	286	0	168	1572	0	0	895	408	266	55–63
OTU2706	MK040683	0.04	Roseiflexus castenholzii(T) DSM 13941 (CP000804)	92	Chloroflexi	25	0	1	19	0	0	39	18	20	55–63
OTU4275	MK040710	0.03	Roseiflexus castenholzii(T) DSM 13941 (CP000804)	91	Chloroflexi	8	63	3	0	15	2	0	0	4	37–60
OTU1134	MK040650	0.05	Roseiflexus sp. RS‐1 (CP000686)	91	Chloroflexi	93	0	9	32	0	0	0	0	0	55–59
OTU2101	MK040676	0.21	Uncultured Anaerolinea sp. clone AE1b_G7 (KC211795)	94	Chloroflexi	0	0	0	0	0	0	0	233	354	59–60
OTU819	MK040720	0.22	Uncultured bacterium clone AKIW403 (DQ129386)	90	Chloroflexi	0	0	0	0	179	424	0	0	0	37–50
OTU2402	MK077657	0.06	Uncultured bacterium clone B25 (AF407718)	100	Chloroflexi	69	0	96	0	0	0	0	1	0	55–59
OTU939	MK077670	0.03	Uncultured bacterium clone B25r (KJ766177)	95	Chloroflexi	0	88	0	0	0	0	0	0	0	42
OTU3240	MK077662	0.03	Uncultured bacterium clone BBL‐OTU64 (JQ791637)	88	Chloroflexi	3	71	0	0	0	0	0	0	0	42–49
OTU4012	MK040708	0.05	Uncultured bacterium clone FCPN412 (EF516361)	89	Chloroflexi	0	0	0	0	148	0	0	0	0	50
OTU1433	MK040661	0.07	Uncultured bacterium clone SM2G06 (AF445738)	98	Chloroflexi	24	0	173	4	0	0	0	0	0	55–59
OTU126	MK040655	0.53	Uncultured bacterium clone Tat‐08‐003_12_54 (GU437328)	97	Chloroflexi	932	1	10	89	0	0	53	118	260	42–63
OTU139	MK077651	0.11	Uncultured bacterium clone Tat‐08‐003_12_54 (GU437328)	96	Chloroflexi	180	0	0	23	0	0	15	29	69	59–63
OTU17	MK040624	0.62	Uncultured Chloroflexi bacterium clone DTB125 (EF205529)	94	Chloroflexi	0	0	0	0	0	1	1,155	438	132	37–63
OTU3490	MK077663	0.03	Uncultured Chloroflexi bacterium clone IAFpp7112 (GU214126)	93	Chloroflexi	0	0	0	0	0	0	0	0	82	60
OTU78	MK077668	0.39	Uncultured Chloroflexi bacterium clone IAFpp722 (GU214145)	98	Chloroflexi	0	5	1,062	31	0	0	0	0	2	42–60
OTU559	MK077664	0.07	Uncultured Chloroflexi bacterium clone OTU52 (HQ416798)	97	Chloroflexi	0	0	0	0	0	0	1	111	75	59–63
OTU562	MK077665	0.07	Uncultured Chloroflexi bacterium clone Pink_D09 (GQ483857)	91	Chloroflexi	0	0	1	2	0	0	0	15	173	55–60
OTU142	MK077652	0.07	Uncultured Chloroflexi bacterium clone QEDN8AA01 (CU926200)	94	Chloroflexi	25	3	46	29	7	1	18	46	7	37–63
OTU3007	MK040686	0.25	Uncultured Chloroflexus sp. clone: 20‐91‐ArvAB (AB425067)	91	Chloroflexi	5	643	9	4	0	0	0	0	41	42–60
OTU103	MK077649	0.43	Uncultured Kouleothrix sp. clone M2‐008 (KF183047)	98	Chloroflexi	0	1,111	3	0	0	89	0	0	0	37–55
OTU889	MK040721	0.06	Uncultured soil bacterium clone 1_D9 (EU589265)	95	Chloroflexi	5	126	41	1	0	0	0	0	0	42–59
OTU1605	MK040670	0.04	Ancylothrix terrestris 13PC (KT819202)	95	Cyanobacteria	2	0	0	0	100	4	0	0	0	37–50
OTU71	MK040625	6.19	Ancylothrix terrestris 13PC (KT819202)	98	Cyanobacteria	4	4	0	0	17,063	153	0	0	10	37–60
OTU12	MK040653	8.8	Chlorogloeopsis sp, Greenland_5 (DQ431000)	98	Cyanobacteria	1	0	7	24,493	0	0	0	0	0	55–59
OTU3380	MK040693	0.04	Cyanothece sp. 2.6 (KJ654305)	97	Cyanobacteria	0	73	31	0	0	0	0	0	0	42–55
OTU1171	MK040651	0.08	Fischerella sp. MV11 (DQ786169)	97	Cyanobacteria	60	11	20	8	1	0	0	37	87	42–60
OTU134	MK040656	24.9	Fischerella sp. MV11 (DQ786169)	99	Cyanobacteria	5,700	577	14,721	3,317	1842	5	0	11,323	31,859	37–60
OTU1553	MK040669	0.04	Fischerella sp. MV11 (DQ786169)	95	Cyanobacteria	63	7	0	0	0	0	0	3	27	42–60
OTU163	MK040671	0.07	Fischerella sp. MV11 (DQ786169)	94	Cyanobacteria	74	0	1	1	2	1	0	8	117	37–60
OTU2353	MK040680	0.04	Fischerella sp. MV11 (DQ786169)	92	Cyanobacteria	16	0	12	10	0	0	0	10	60	55–60
OTU366	MK040696	0.15	Fischerella sp. MV11 (DQ786169)	96	Cyanobacteria	297	21	3	0	0	0	0	14	79	42–60
OTU763	MK040718	0.05	Fischerella sp. MV11 (DQ786169)	95	Cyanobacteria	0	0	129	0	0	0	0	0	0	55
OTU790	MK040719	0.12	Fischerella sp. MV11 (DQ786169)	95	Cyanobacteria	21	33	63	61	20	0	0	36	99	42–60
OTU48	MK040639	0.28	Leptolyngbya O77 (AP017367)	97	Cyanobacteria	2	0	2	0	0	0	0	33	736	55–60
OTU85	MK040644	0.21	Leptolyngbya ramosa PUPCCC (KM376988)	97	Cyanobacteria	0	0	230	0	0	9	303	41	1	37–63
OTU110	MK040649	3.61	Leptolyngbya sp, BX10 (HM151385)	98	Cyanobacteria	2	0	0	0	2,854	7,186	0	0	4	37–60
OTU3263	MK040689	0.04	Leptolyngbya sp. LEGE 07319 (HM217045)	94	Cyanobacteria	0	0	5	0	1	0	0	5	105	50–60
OTU3974	MK040704	0.05	Leptolyngbya sp. LEGE 07319 (HM217045)	91	Cyanobacteria	0	141	0	0	0	0	0	0	0	42
OTU3318	MK040691	0.09	Limnothrix redekei CCAP 1459/29 (HE974998)	96	Cyanobacteria	0	0	0	0	173	65	0	0	0	37–50
OTU124	MK040654	3.81	Limnothrix sp, B15 (GQ848190)	98	Cyanobacteria	3	1,037	0	0	266	9,295	0	0	0	37–50
OTU2610	MK040682	0.05	Limnothrix sp, B15 (GQ848190)	96	Cyanobacteria	0	140	0	0	0	1	0	0	0	37–42
OTU117	MK040631	0.95	Limnothrix sp, CENA545 (KF246506)	96	Cyanobacteria	0	0	0	0	16	2,622	0	0	0	37–50
OTU93	MK040642	0.07	Lyngbya wollei (EU603708)	97	Cyanobacteria	0	48	148	0	0	0	0	0	0	42–55
OTU3444	MK040694	0.03	Lyngbya wollei (EU603709)	92	Cyanobacteria	0	32	59	0	0	0	0	0	0	42–55
OTU296	MK040685	0.09	Microcoleus sp. PCC 7113 (CP003630)	94	Cyanobacteria	0	0	260	0	0	0	0	1	0	55–59
OTU2714	MK040684	0.11	Oscillatoriales cyanobacterium JSC‐1 (FJ788926)	99	Cyanobacteria	0	0	309	2	0	0	0	0	3	55–60
OTU1462	MK040636	0.04	Phormidium animale SAG 1459‐6 (EF654087)	92	Cyanobacteria	0	0	0	0	60	51	0	0	1	37–60
OTU1537	MK040667	0.15	Phormidium sp, DVL1003c (JQ771628)	96	Cyanobacteria	0	0	0	0	49	371	0	0	0	37–50
OTU0	MK040626	0.67	Synechococcus lividus C1 (AF132772)	99	Cyanobacteria	0	0	938	380	0	0	2	355	199	55–63
OTU21	MK040675	0.63	Synechococcus sp, JA‐3‐3Ab genotype A‐NACy05a (AY884052)	96	Cyanobacteria	0	0	0	0	0	0	1737	17	4	59–63
OTU141	MK040658	13.81	Uncultured bacteriumclone: B1001R003_P01 (AB659771)	97	Cyanobacteria	2	37,959	503	2	0	0	0	0	1	42–60
OTU99	MK040726	0.08	Uncultured bacteriumclone: B1001R003_P01 (AB659771)	94	Cyanobacteria	0	214	3	0	0	0	0	0	0	42–55
OTU140	MK040634	5.16	Uncultured Oscillatoriales cyanobacterium clone E3‐00YK9 (EU376433)	98	Cyanobacteria	10,539	44	2	10	668	0	0	518	2,584	42–60
OTU25	MK077658	0.03	Uncultured Oscillatoriales cyanobacterium clone H_10 (FJ490330)	86	Cyanobacteria	0	0	95	0	0	0	0	0	0	55
OTU3924	MK040702	0.03	Uncultured Firmicutes bacterium clone D2D09 (EU753609)	91	Firmicutes	0	0	1	0	0	0	0	90	0	55–59
OTU127	MK040628	0.05	Uncultured bacterium clone Drod‐B13 (FJ206764)	99	Planctomycetes	10	0	0	0	0	0	0	34	95	59–60
OTU1271	MK077650	0.04	Uncultured bacterium clone Drod‐B45 (FJ206785)	89	Planctomycetes	0	0	0	0	0	0	0	6	94	59–60
OTU3173	MK077661	0.03	Uncultured bacterium isolate 1112865250968 (HQ119290)	85	Planctomycetes	0	0	0	0	0	0	0	7	83	59–60
OTU1829	MK040673	0.04	Altererythrobacter dongtanensis JM27(T) (GU166344)	97	Proteobacteria	0	11	91	0	1	0	0	0	0	42–55
OTU118	MK040652	0.1	Elioraea tepidiphila DSM 17972(T) (KB899943)	98	Proteobacteria	0	0	127	21	0	0	0	61	58	55–60
OTU1452	MK040663	0.04	Erythrobacter sp. 5IX/A01/140 (AY576736	98	Proteobacteria	0	25	74	14	9	1	0	0	1	37–60
OTU132	MK040633	0.08	Haliangium tepidum SMP‐10(T) (AB062751)	92	Proteobacteria	0	233	1	0	0	0	0	0	0	42–55
OTU227	MK040679	0.06	Hydrogenophaga defluvii strain BSB 9.5(T) (NR029024)	95	Proteobacteria	0	134	23	0	0	0	0	0	0	42–55
OTU4289	MK040711	0.05	KY386562 Polymorphobacter sp. strain R‐68699 (KY386562)	95	Proteobacteria	0	0	2	1	0	0	0	11	114	55–60
OTU31	MK040687	0.14	Lacibacterium aquatile LTC‐2(T) (HE795994)	92	Proteobacteria	0	384	2	0	0	0	0	0	0	42–55
OTU1068	MK040647	0.04	Leptothrix mobilis strain Feox‐1 DSM10617(T) (NR026333)	97	Proteobacteria	0	0	1	0	0	122	0	0	0	37–55
OTU1471	MK040664	0.03	Lysobacter thermophilus strain YIM 77875 (JQ746036)	99	Proteobacteria	0	1	73	0	0	0	0	0	0	42–55
OTU108	MK040629	0.07	Piscinibacter defluvii SH‐1(T) (KU667249)	98	Proteobacteria	0	194	0	0	1	13	0	0	0	37–50
OTU3722	MK040699	0.15	Polyangium spumosum strain Pl sm5 (GU207881)	94	Proteobacteria	0	416	0	0	1	0	0	0	0	42–50
OTU3822	MK040700	0.06	Porphyrobacter cryptus ALC‐2 (T) (AF465834)	99	Proteobacteria	0	47	102	11	1	2	0	0	3	37–60
OTU3955	MK040703	0.03	Pseudorhodoplanes sinuspersici strain RIPI 110 (NR145909)	97	Proteobacteria	0	15	51	0	6	0	0	1	1	42–60
OTU1373	MK040657	0.03	Rubritepida flocculans DSM 14296(T) (AF465832)	98	Proteobacteria	0	0	59	15	0	0	0	0	0	55–59
OTU3321	MK040692	0.14	Salinarimonas ramus strain SL014B‐41A4 (NR108683)	95	Proteobacteria	0	0	393	0	0	0	0	0	0	55
OTU1	MK040627	0.08	Tabrizicola aquatica strain RCRI19(T) (HQ392507)	99	Proteobacteria	0	167	43	0	0	2	0	0	0	37–55
OTU1542	MK040668	0.05	Tepidimonas taiwanensis I1‐1(T) (AY845054)	98	Proteobacteria	0	0	0	0	0	0	0	41	90	59–60
OTU3716	MK040698	0.04	Thermophilic methanotroph HB (U89299)	92	Proteobacteria	0	0	0	0	0	0	38	11	60	59–63
OTU101	MK040645	0.15	Uncultured bacterium clone JulG‐B86 (FJ206635)	96	Proteobacteria	0	0	10	134	0	0	97	111	65	55–63
OTU464	MK040713	0.03	Uncultured bacterium clone kab116 (FJ936833)	95	Proteobacteria	38	0	0	1	0	0	1	24	17	59–63
OTU1092	MK040648	0.03	Uncultured bacterium clone NC24c1_18286 (JQ368669)	88	Proteobacteria	0	0	0	0	78	0	0	0	0	50
OTU907	MK040723	0.03	Uncultured bacterium clone: B1001R003_P01.(AB659771)	94	Proteobacteria	0	74	0	0	0	0	0	0	0	42
OTU609	MK077667	0.03	Uncultured bacterium partial clone RNB‐C147 (LN680248)	92	Proteobacteria	0	0	0	0	0	0	42	18	14	59–63
OTU96	MK040643	0.14	Uncultured beta proteobacterium clone Aug‐CD266 (JQ795254)	96	Proteobacteria	7	65	22	2	1	2	0	2	290	37–60
OTU6	MK040640	0.07	Uncultured Haliangium sp. clone Pad‐72 J (X505319)	98	Proteobacteria	0	0	201	0	0	0	0	0	0	55
OTU3992	MK040705	0.07	Uncultured Rhodocyclaceae bacterium clone Elev_16S_555 (EF019343)	91	Proteobacteria	0	168	1	0	2	15	0	0	0	37–55
OTU3272	MK040690	0.04	Uncultured bacterium clone FFCH13324 (EU135381)	92	Saccharibacteria	0	0	0	0	2	107	0	0	0	37–50
OTU1458	MK077653	0.04	Leptonema illini DSM 21528 (JH597773)	82	Spirochaetes	0	0	0	120	0	0	0	0	0	59
OTU2227	MK040678	0.05	Meiothermus hypogaeus AZM34c11(T) (AB586707)	96	Deinococcus‐Thermus	0	0	0	0	0	0	0	24	121	60
OTU41	MK040638	0.03	Meiothermus hypogaeus AZM34c11(T) (AB586707)	97	Deinococcus‐Thermus	1	0	40	4	0	0	0	0	30	55–60
OTU49	MK040715	0.32	Meiothermus ruber DSM 1,279(T) (CP001743)	95	Deinococcus‐Thermus	0	0	26	609	144	1	41	56	4	37–63
OTU957	MK040725	0.38	Meiothermus ruber DSM 1279(T) (CP001743)	99	Deinococcus‐Thermus	0	0	6	102	0	0	783	151	22	55–63
OTU1443	MK040662	0.16	Meiothermus terrae YIM 77755(T) (KF603888)	98	Deinococcus‐Thermus	0	0	435	5	0	0	0	0	0	55–59
OTU4	MK040706	0.71	Thermus oshimai strain SPS‐17(T) (Y18416)	97	Deinococcus‐Thermus	0	0	0	0	0	0	1978	11	2	59–63

^aWhen possible, we use only published or type strain reference sequences to compare with OTU sequences from this work.

^bRío Negro (RN), Miravalles (MV), Bajo las Peñas (BP), Rocas Calientes (RC).