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. 2019 Oct 18;10:2384. doi: 10.3389/fimmu.2019.02384

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Copyright © 2019 Leite Pereira, Bitoun, Paoletti, Nocturne, Marcos Lopez, Cosma, Le Grand, Mariette and Tchitchek.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Monocytes, cDCs, and pDCs from RA patients respond normally to TLR stimulation. Whole blood cells from healthy donors and RA patients were stimulated for 2 h with a mixture of TLR ligands. Controls were performed using PBS. The staining of cytokines was performed intracellularly. Monocyte, cDC, and pDC populations were exported from the SPADE analysis represented in Supplementary Figure 1. The percentage of (A) monocytes, (B) cDCs, and (C) pDCs producing MIP-1β, TNF-α, and IL-8 after stimulation are presented. Blue points correspond to the percentages of cells obtained from healthy subjects, red points to those from RA patients.