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. 2019 Oct 18;10:2436. doi: 10.3389/fimmu.2019.02436

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Copyright © 2019 Dusi, Angiari, Pietronigro, Lopez, Angelini, Zenaro, Della Bianca, Tosadori, Paris, Amoruso, Carlucci, Constantin and Rossi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dynamics of Th1 and Th17 cell movement inside the spinal SAS at the EAE disease peak. Exogenous myelin-specific autoreactive Th1 and Th17 cells were injected into EAE mice at the disease peak (11–13 dpi). TPLM imaging was performed on the exposed spinal cord 48 h later. The mean velocity (A), meandering index (B) and arrest index (C) were calculated to compare the behavior of infiltrating cells. Data represent the mean ± SEM from a minimum of nine to a maximum of 490 cells from three independent experiments (^***P < 0.0001). (D) Comparison of the dynamic behavior of the Th1 and Th17 populations. Data represent the mean ± SEM of six different fields from three independent experiments.