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. 2019 Oct 18;10:2436. doi: 10.3389/fimmu.2019.02436

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Copyright © 2019 Dusi, Angiari, Pietronigro, Lopez, Angelini, Zenaro, Della Bianca, Tosadori, Paris, Amoruso, Carlucci, Constantin and Rossi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Integrin expression on Th1 and Th17 cells. The expression of α4 integrins (A) and LFA-1 (B) on Th1 and Th17 was evaluated both in vitro before injection and ex vivo on exogenous CMAC⁺ T cells isolated from spinal cords 48 h after adoptive transfer. Samples were analyzed by flow cytometry. The mean fluorescence intensity (MIF) of LFA-1 was statistically higher on Th1 cells than Th17 cells both before injection and 48 h after cell transfer. Data represent the mean ± SD of four mice per condition from two independent experiments (^*P < 0.05).