FIGURE 11.
Effect of oxygen in competition, antibiotic and bacteriocin production by Pectobacterium carotovorum subsp. brasiliense 1692. (A) Carbapenem production by Pcb1692 strains spotted on a lawn of PcbG4P5 and incubated aerobically and anaerobically. (B) Bacteriocin production by Pcb1692 strains induced with mitomycin C and overlaid with Bacillus cereus. (C) Interbacterial competition assays between Pcb1692 and the targeted strain PcbG4P5, data is presented as CFU/ml of recovered targeted bacteria following 24 h incubation aerobically and anaerobically. Halo area was measured using ImageJ and data presented as the mean, error bars represent one standard deviation and asterisk represent statistical significant difference (p < 0.05) as determined by the two-tailed Student’s t-test. Experiments were performed in triplicates and repeated three independent times.