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. 2019 Oct 18;10:2379. doi: 10.3389/fmicb.2019.02379

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Copyright © 2019 Shyntum, Nkomo, Shingange, Gricia, Bellieny-Rabelo and Moleleki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Iron is required for production of carbapenem by Pectobacterium carotovorum subsp. brasiliense 1692. Pcb1692 wild-type mutant and complemented mutant strains were spotted on a lawn of P. atrosepticum and clear zone around the spotted Pcb strains was indicative of carbapenem production. (A) Carbapenem production on LB agar by Pcb strains. (B) No detectable levels of carbapenem were observed on M9. (C) Halo area was measured using ImageJ and data presented as the mean, error bars represent one standard deviation and asterisk represent statistical significant difference (p < 0.05) as determined by the two-tailed Student’s t-test. Experiments were performed in triplicates and repeated three independent times.