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. 2019 Oct 18;10:2379. doi: 10.3389/fmicb.2019.02379

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Copyright © 2019 Shyntum, Nkomo, Shingange, Gricia, Bellieny-Rabelo and Moleleki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bacteriocin production by strains of Pectobacterium carotovorum subsp. brasiliense 1692. Pcb1692 and its isogenic mutant strains were spotted on LB and grown overnight. (A) Bacteriocin production was induced with mitomycin C (B) control plate not induced. Dickeya dadantii was used as the indicator strain. Clear zones are indicative of bacteriocin production by Pcb1692. (C) Halo area was measured and ImageJ and data presented as the mean, error bars represent one standard deviation and asterisk represent statistical significant difference (p < 0.05) as determined by the two-tailed Student’s t-test. Experiments were performed in triplicates and repeated three independent times. Ns, not statistically significant.