FIGURE 6.

Bovine lactoferrin digest eDNA in preformed Streptococcus pneumoniae biofilms. S. pneumoniae strain D39 (7.5 × 10⁴ CFU/ml) was inoculated in a 24-well plate containing THY and incubated for 4 h (A) or 8 h (B) at 37°C with a 5% CO₂ atmosphere. Planktonic cells were removed; biofilms washed once and then treated with 40 μM bLF, 10 μM, LFcin17-30, 10 μM LFampinn265-284, and 10 μM LFchimera for 6 h (A) or 12 h (B). After that, the supernatants were removed and DNA extracted. eDNA quantification was performed by using qPCR. Experiments were repeated at least three times. Standard deviation of the mean is shown. Asterisks indicate statistical significance calculated using the ANOVA test (p < 0.05).