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. 2019 Oct 18;10:2386. doi: 10.3389/fmicb.2019.02386

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Copyright © 2019 Angulo-Zamudio, Vidal, Nazmi, Bolscher, Leon-Sicairos, Antezana, Canizalez-Roman and León-Sicairos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bovine lactoferrin degrades eDNA from antibiotic-resistance strains of Streptococcus pneumoniae. S. pneumoniae strains TIGR4^Tmp (A), GA47281^Tet+Ery+Cm (B), and GA44194^Tet+Ery+Cm (C) were inoculated in a 24-well plate containing THY. Then, the plate was incubated for 4 h at 37°C with a 5% CO₂ atmosphere. Planktonic cells were removed, biofilms washed once, and bovine Lactoferrin (bLF) was added at the indicated concentrations and incubated for 6 h. The supernatants were removed, filter-sterilized to eliminate bacteria in supernatant and DNA was extracted. eDNA quantification was performed by using qPCR. Experiments were repeated at least three times. Standard deviation of the mean is shown. Asterisks indicate statistical significance calculated using the ANOVA test (p < 0.05).