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Signaling properties of LmMC4R. HEK293T cells were transiently transfected with hMC4R or LmMC4R plasmids. Forty-eight hours after transfection, different concentrations of NDP-MSH (A), α-MSH (B), ACTH (1-24) (C), and THIQ (D) was used to stimulate the cells transfected with hMC4R or LmMC4R. Data are expressed as mean ± SEM from triplicate measurements within one experiment. The curves are representative of three independent experiments.
