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The toxicity of the fraction results from column chromatography.
| Sample | Concentration (ppm) | Number of dead larvae | Mortality (%) | LC50 (ppm) | ||
|---|---|---|---|---|---|---|
| I | II | III | ||||
| FA | 0 | 0 | 0 | 0 | 0 | 50.12 |
| 10 | 2 | 1 | 1 | 7 | ||
| 100 | 7 | 7 | 6 | 67 | ||
| 1000 | 10 | 9 | 9 | 94 | ||
| FB | 0 | 0 | 0 | 0 | 0 | 44.67 |
| 10 | 2 | 1 | 1 | 8 | ||
| 100 | 7 | 7 | 6 | 73 | ||
| 1000 | 10 | 10 | 9 | 100 | ||
| FC | 0 | 0 | 0 | 0 | 0 | 56.23 |
| 10 | 2 | 2 | 1 | 13 | ||
| 100 | 7 | 6 | 6 | 62 | ||
| 1000 | 10 | 9 | 9 | 94 | ||
| FD | 0 | 0 | 0 | 0 | 0 | 79.43 |
| 10 | 2 | 2 | 1 | 13 | ||
| 100 | 5 | 5 | 6 | 54 | ||
| 1000 | 10 | 9 | 9 | 94 | ||
FA=Fraction A, FB=Fraction B, FC=Fraction C, FD=Fraction D
