Table 2.
The results are presented as log10 CFU/ml (mean±SE) of Salmonella infantis and E. coli inactivating efficacy, using 0.17% and 3% of food additive grade calcium hydroxide solution, in the absence or presence of organic materials.
| Conditions | Salmonella infantis | E. coli | ||||||
|---|---|---|---|---|---|---|---|---|
| 0.17% | 3% | 0.17% | 3% | |||||
| 0% FBS | 5% FBSa | 0% FBS | 5% FBS | 0% FBS | 5% FBS | 0% FBS | 5% FBS | |
| tpcb | 8.65±0.19 | 8.65±0.19 | 8.65±0.19 | 8.65±0.19 | 9.10±0.19 | 9.10±0.19 | 9.10±0.19 | 9.10±0.19 |
| 0 sc | 8.85±0.51 | 8.70±0.17 | 8.67±0.20 | 8.69±0.14 | 9.09±0.45 | 8.84±0.25 | 8.94±0.31 | 8.75±0.32 |
| 1 mind | 6.73±0.66 | 6.66±0.52 | 6.38±0.38 | 6.30±0.48 | 7.60±0.00 | 7.60±0.00 | 7.60±0.00 | 7.44±0.29 |
| 3 min | 2.70±0.17* | 3.25±0.75* | 3.38±1.34* | 3.51±1.58* | 6.57±0.49 | 6.96±0.36 | 4.51±0.27* | 5.31±0.41* |
| 5 min | 2.60±0.00* | 2.70±0.17* | 2.60±0.00* | 2.60±0.00* | 3.81±1.05* | 4.24±1.44* | 2.60±0.00* | 2.60±0.00* |
Fetal bovine serum was added to FdCa (OH)2 solution so as to represent 5% organic materials of total volume.
The titer converted into an index in log10 of bacteria control.
The titer in log10 that added blocking solution before Salmonella infantis or E. coli.
The titer converted into an index in log10 of the recovered bacteria after indicated time of treatment, such as 1 min, 3 min, and 5 min.
Inactivation regarded as effective when RF was ≥3. E. coli=Escherichia coli, RF=Reduction factor, CFU=Colony-forming units, tpc=Titer of bacteria/virus control, FBS=Fetal bovine serum, FdCa (OH)2=Food additive grade calcium hydroxide