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. 2019 Oct 18;10:1143. doi: 10.3389/fpls.2019.01143

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Copyright © 2019 Rahim, Resentini, Dalla Vecchia and Trainotti

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detailed type I MYB10.1 phenotype during flower development. (A) Alteration of floral development in type I compared to (C) WT (stages were defined according to Koltunow et al. (1990). Bars = 200 pixel. (B) Cleared sections in wild-type developing ovules from stage st3-I to st3-V, the seven-celled embryo sac. (E) In ovules of overexpressing lines, the development is blocked to st3-I. (D) Thin sections of WT and type I ovules confirm the developmental arrest. (F) Aniline blue staining evidenced the persisting deposition of callose in pollinated type I plants, while the callose disappears in pollinated wild-type flowers. Ovules stages are according to Schneitz et al. (1995). ap, antipodal cells; cc, central cell; ec, egg cell; fg, FG; ii, inner integument; oi, outer integument; syn, synergid cells; v, vacuole. Bars are 1 cm in A and B; 50 µm in B, D, E, F St3-I; 200 µm in F St3-V.