Table 2.
In vitro experimental design.
| Experimental model | Experiments | DIVl | DIV3 | DIV11 | DIV14 | DIV18 |
|---|---|---|---|---|---|---|
| WT neurons | Immunocytochemistry (n = 12) | Seeded neurons | lmmunocytochemistry (MAP2 GFAP) | |||
| Living cell imaging (n = 12) | Transfected LV-EGFP-CLSTN1 LV-ICAM5-mCherry | Time lapse (ICAM5 CLSTN1) | ||||
| Western blotting (n = 12) | Transfected LV-Cl.STN1-shRNA | Western blotting (CLSTN1 ICAM5 Psd95) | ||||
| Immunocytochemistry (n = 12) | lmmunocytochemistry (ICAM5 Psd95) | |||||
| Dil-stained (n = 12) | Dil-stained (dendritic spines) | |||||
| Fmrl KO neurons | Immunocytochemistry (n = 12) | Transfected LV-EGFP-CLSTN1 | Immunocytochemistry (ICAM5 CLSTN1) | |||
| Western blotting (n = 12) | Western blotting (CLSTN1 ICAM5 Psd95) | |||||
| Dil-stained (n = 12) | Dil-stained (dendritic spines) | |||||
| N2A | Immunocytochemistry (n = 8) | Immunocytochemistry (ICAM5 Cl.STN1) |
DIV, days in vitro.