Table 2.

Applications of affinity chromatography and HPAC in kinetic studies^a.

Technique	System examined
Plate height method	Drug and solute binding with serum proteins (HSA); sugar-lectin interactions (Con A)
Peak profiling	Drug, drug metabolite, and solute binding with serum proteins (AGP, HSA); drug binding with cyclodextrins; drug-receptor interactions (β2-AR)
Peak decay	Drug binding with serum proteins (AGP, HSA); sugar-lectin interactions (Con A); antibody-antigen binding; aptamer-target interactions; interactions of immunoglobulins with protein A or protein G
Split-peak method	Antibody-antigen binding; interactions of immunoglobulins with protein A or protein G
Peak fitting in zonal elution	Sugar-lectin interactions (Con A); drug/inhibitor-receptor interactions (nAChR, β2-AR); drug binding with cyclodextrins; interactions of immunoglobulins with protein A; binding of novobiocin with heat shock protein; lysozyme binding with Cibacron Blue 3GA
Peak fitting in frontal analysis	Sugar-lectin interactions (Con A); antibody-antigen binding
Ultrafast affinity extraction	Drug and hormone binding with serum proteins (AGP, HSA, SHBG);

^aAGP, α₁-acid glycoprotein; β₂-AR, beta₂-adrenoceptor; Con A, concanavalin A; HSA, human serum albumin; nAChR, nicotinic acetylcholine receptor; SHBG, sex-hormone binding globulin.