Figure 2.

Structures of PSMA conjugates, the photonic properties of LC-Pyro, and the radio-HPLC trace of ⁶⁴Cu-LC-Pyro. (A) Structures of LC-Pyro (Long-circulating pyropheophorbide a), SC-Pyro (Short-circulating pyropheophorbide a) and DCIBzL. (B) LC-Pyro absorbance spectrum and (C) fluorescence emission spectrum. (D) Reactive oxygen species generation from LC-Pyro in solution with respect to 671 nm laser light dose (n = 3 from three independent measurements). (E) Evaluation of radiochemical purity of ⁶⁴Cu-labeled LC-Pyro by radio-HPLC monitored by absorption at 410 nm (left, LC-Pyro signal) and by radioactive detector (⁶⁴Cu signal). The only peak at a retention time of 11 min showed both 410 nm and radioactive signal indicated high purity of ⁶⁴Cu-labeled LC-Pyro.