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. Author manuscript; available in PMC: 2019 Oct 25.
Published in final edited form as: Bioconjug Chem. 2018 Oct 29;29(11):3746–3756. doi: 10.1021/acs.bioconjchem.8b00636

Figure 3.

Figure 3.

PSMA targeting selectivity and specificity in vitro. (A) Western blots of PC3, PSMA- PC3 flu, PSMA+ PC3 PIP, PC3- ML-1117, and PC3- ML-1124 cell lysates using anti-PSMA and β-actin antibodies. (B) Representative fluorescence micrographs of LC-FITC (3 μM, 3 h incubation) uptake in PSMA- PC3 flu and PSMA+ PC3 PIP cells in the presence of 100× excess DCIBzL for target blockade. Scale = 25 μm. (C) Representative flow cytometry histogram plots with time-dependent quantification of LC-FITC uptake in PSMA- PC3 flu (open black square) and PSMA+ PC3 PIP (solid green circle) cells. Each point represents the median ± SD of three independent measurements.