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Peroxidase activity of WT and A51V Hu Cytc. (a) Michaelis-Menten plots for the A51V variant of Hu Cytc at pH values from 5 to 8. The solid curves are fits to eq 4 (Experimental Methods). Data were acquired at 25 °C in 50 mM buffer. (b) kcat versus pH for WT (previously published,45, 64 dark green bars) and A51V (red bars) Hu Cytc. Error bars are the standard deviation from three independent experiments.
