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. Author manuscript; available in PMC: 2020 Oct 24.
Published in final edited form as: J Phys Chem B. 2019 Oct 14;123(42):8939–8953. doi: 10.1021/acs.jpcb.9b05869

Table 4.

Thermodynamic Parameters for the Acidic Transition of WT and A51V Hu Cytc at 22 ± 2 °C

695 nm 622 nm
Variant pH1/2 pKC(A/N)a n pH1/2 pKC(A/N)a n
A51V 3.19 ± 0.09 −1.4 ± 0.3 1.8 ± 0.4 3.13 ± 0.06 −1.1 ± 0.2 1.3 ± 0.1
WTb 2.50 ± 0.03 −2.50 ± 0.07 1.64 ± 0.07 2.41 ± 0.03 −1.73 ± 0.06 1.13 ± 0.08
a

Assumes the pKa of the ionizable group(s) is 4.

b

Parameters are taken from ref64.