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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Brain Behav Immun. 2019 Apr 24;85:46–56. doi: 10.1016/j.bbi.2019.04.031

Fig. 3.

Fig. 3.

NeuN staining to quantify neuronal density in brain tissue in dietary treatment groups at the end of the study. (A) Sample NeuN staining of preterm hippocampus (HC) at 4× and 20× magnification of CA3 section. (B) Sample NeuN staining of preterm parietal cortex (PC) at 4× and 20× magnification. (C) Comparison of visualized neurons per 20× HPF between term piglet and four preterm piglet study groups. The number of tissue sections analyzed in the hippocampus, nucleus ventralis posterior thalamus, parietal cortex and temporal cortex were as follows: Term n = 2–4, ENT n = 3–10, IL n = 8–11, SMOF n = 6–10, EXP n= 5–10. Data are presented as mean ± SEM. * P < 0.01 Term v preterm, ** P = 0.02 Term v IL