Table 3.

Comparison of 5 gene methylation levels and semen quality/hormone level in all subjects.

	Sperm motility (% moving forward)		Concentration (106 spermatozoa/ml)		Sperm vitality (% alive)		FSH		LH		T
	r	P	r	P	r	P	r	P	r	P	r	P
Neither nicotine nor alcohol Exposed
MEST	−0.033	0.828	0.317	0.030	0.121	0.525	0.022	0.886	0.051	0.735	0.025	0.867
P16	−0.024	0.872	0.020	0.894	−0.119	0.524	0.211	0.154	−0.181	0.223	−0.006	0.970
H19	−0.276	0.061	−0.147	0.323	−0.373	0.039	0.263	0.078	0.177	0.240	0.027	0.857
LINE1	−0.101	0.496	−0.224	0.125	−0.171	0.357	0.071	0.635	−0.059	0.695	−0.040	0.789
GNAS	−0.171	0.246	−0.248	0.090	−0.199	0.283	0.081	0.586	0.120	0.420	0.072	0.628
Alcohol exposed only
MEST	0.082	0.763	−0.522	0.038	0.024	0.955	−0.143	0.626	0.262	0.366	−0.389	0.169
P16	−0.114	0.674	−0.291	0.274	0.582	0.130	0.026	0.930	0.333	0.245	0.019	0.949
H19	−0.284	0.286	−0.350	0.184	0.306	0.461	−0.266	0.359	0.207	0.478	−0.089	0.761
LINE1	0.169	0.531	0.016	0.953	0.518	0.188	−0.082	0.780	0.113	0.701	−0.448	0.108
GNAS	−0.054	0.843	−0.557	0.025	−0.005	0.986	−0.207	0.478	0.176	0.547	0.044	0.881
Nicotine exposed only
MEST	0.041	0.884	−0.474	0.075	−0.333	0.347	0.088	0.745	−0.044	0.871	−0.362	0.169
P16	−0.354	0.196	−0.104	0.712	0.034	0.925	−0.108	0.692	−0.327	0.216	0.219	0.415
H19	−0.134	0.633	−0.427	0.113	0.604	0.064	−0.076	0.778	−0.071	0.795	−0.062	0.820
LINE1	0.056	0.849	0.041	0.890	−0.240	0.534	0.046	0.869	0.175	0.532	−0.122	0.664
GNAS	0.382	0.160	0.062	0.825	−0.544	0.104	0.209	0.438	0.482	0.058	−0.358	0.173
Both nicotine and alcohol exposed
MEST	−0.062	0.648	−0.196	0.144	−0.023	0.889	0.241	0.082	0.344	0.012	0.016	0.910
P16	0.064	0.631	0.072	0.589	−0.050	0.761	0.097	0.481	0.019	0.892	−0.074	0.593
H19	−0.140	0.295	−0.103	0.441	−0.065	0.685	−0.059	0.673	0.024	0.864	0.184	0.186
LINE1	−0.037	0.780	−0.077	0.561	−0.067	0.676	0.436	0.001	0.173	0.206	0.168	0.221
GNAS	−0.187	0.159	−0.218	0.101	−0.087	0.593	0.194	0.156	0.365	0.006	0.066	0.630

The bold represent p<0.05.