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. 2019 Oct 24;14:118. doi: 10.1186/s13000-019-0887-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Impact of CPXM2 on the proliferation and metastasis of osteosarcoma cells in vitro. a Western blotting to examine the EMT process in the hFOB.1.19 cell line and the corresponding statistical analysis of EMT process components. b Growth curves of hFOB.1.19 cells as determined using the Cell Counting kit-8. c The abilities of hFOB.1.19 cells to form colonies under 2D culture condition were determined through colony formation assay. d Matrigel invasion assay to examine the invasive ability of hFOB.1.19 cells in vitro. Scale bars, 20 μm. e Wound-healing assay to detect the migration ability of hFOB.1.19 cells in vitro. ^**P < 0.01 vs. the empty vector groups. CPXM2, Carboxypeptidase X, M14 family member 2; EMT, epithelial mesenchymal transition; OD, optical density