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. Author manuscript; available in PMC: 2020 Aug 22.
Published in final edited form as: Mol Cell. 2019 Aug 22;75(4):700–710.e6. doi: 10.1016/j.molcel.2019.07.032

Figure 5. MORC-1 foci topologically entrap DNA.

Figure 5.

Representative kymographs of Cy3-MORC-1 foci movement on quantum dot labeled DNA (top panel, [MORC-1] = 5 nM, [ATP] = 2 mM) and on bare DNA (bottom panel, [MORC-1] = 10 nM, [ATP] = 2 mM). Schematics below the kymographs illustrate the experiment. MORC-1 is applied to the flow cell and allowed to form foci, then subjected to a 500 mM NaCl and 2 mM MgCl2 wash in the presence of 2 mM ATP. Scale bars represent 20 seconds and 5 μm. SYTOX Orange stained DNA is shown to the right of the kymographs to indicate approximate location of foci on the DNA. Note that the quantum dot is not visible any of the displayed spectral channels. In the top panel, the top focus (magenta asterisk) contains approximately 2 MORC-1s and the bottom focus (lavender asterisk) contains approximately 7 MORC-1s. In the bottom panel, the time gap between the two events as marked by the dashed line is 7.2 seconds. The top focus (orange asterisk) has approximately 7 MORC-1s and the bottom (peach asterisk) has approximately 13 MORC-1s. See also Figure S6, and Videos S4, S5, and S6.