Fig. 4: MBV upregulate Arginase expression independent of Stat6 phosphorylation.
(A, B) BMDM harvested from wt or st2−/− mice were untreated (ctrl), or stimulated for 24 hr (A) or 30 min (B) with IL4, recombinant IL-33, IL33+ MBV, or IL33- MBV). Cell lysates were analyzed by immunoblot for Arginase-1 expression (A) and phosphorylation of Stat6 (B). Activation of the canonical IL-33 signaling cascade was analyzed by immunoblot for p38 and p65 phosphorylation (B).