Table 4.
Number of T1 progeny with indicated genotypes from four transgenic wheat plants that contained Cas9 and sgRNA pairs for TaNFXL1
| Genotype | Number of T1 progeny from plant: | |||
|---|---|---|---|---|
| NFXL1_1 | NFXL1_2 | NFXL1_3 | NFXL1_4 | |
| AXXYY BXXYY DXXYYa | 3 | 9 | 5 | 13 |
| Ax2x2y1y1 Bx2x2y2y2 Dx1x2Yy2 | 6 | |||
| Ax2x2y1y1 Bx2x2y2y2 Dx2x2Yy2 | 4 | |||
| Ax1x2y1y1 Bx1x1y1y2 Dx2x3Yy1y2 | 2 | |||
| AXx3YY BXx4YY DXXYY | 2 | |||
| AXx3YY BXXYY DXXYY | 2 | |||
| AXXYy7 BXXYY DXXYY | 5 | |||
| Other individual unique patterns | 8 | 7 | 15 | 2 |
| Incomplete | 2 | 3 | 0 | 4 |
aGenotype code: A, B and D: wheat subgenomes; X: the two alleles for the gene X group in each subgenome, with X as the wild type allele in Fielder and xn for an allele with a distinct editing n; Y and y: alleles for the gene Y group. See Additional file 10 for complete genotyping data