Fig. 6.

Voluntary wheel running (VWR) promotes myelination via inhibiting the expression of Wnt3a and Wnt9a. a The mRNA levels of Wnts in the motor cortex of control (Ctrl) and VWR mice. Data are presented as mean ± SEM. n = 6 per group; ^***p < 0.001 vs Ctrl, unpaired Student’s t-test. b Expression of Wnt3a and Wnt9a in HEK293 cells. CM, Cell membrane. c, d Wnt3a- and Wnt9a-treated OPCs immunostained for NG2 (OPCs). As shown, the number of OPCs decreased upon treatment with Wnt3a or Wnt9a. Scale bar = 20 μm. Data are presented as minimum to maximum. n = 7 independent coverslips per condition. ^*p < 0.05 vs Ctrl; **p < 0.01 vs Ctrl; unpaired Student’s t-test. e, f Wnt3a- and Wnt9a-treated OPCs immunostained for MBP (OLs). The average branch length of OLs decreased upon treatment with Wnt3a or Wnt9a. Scale bars = 20 μm. Data are presented as minimum to maximum. n = 7 independent coverslips per condition. ^**p < 0.01 vs Ctrl;^*p < 0.05 vs Ctrl; unpaired Student’s t-test