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. Author manuscript; available in PMC: 2019 Oct 25.
Published in final edited form as: Ann Integr Mol Med. 2019 Aug 22;1(1):23–47.

Figure 2: Detection of 5’-truncated sense strand globin RNA sequences containing 5’-terminal poly(U) segments.

Figure 2:

Uppercase letters – nucleotide sequence of the sense strand; lowercase letters – nucleotide sequence of the antisense strand. Highlighted in green – “AUG” translation initiation codon on the sense strand; highlighted in blue – “uac” complement of translation initiation codon on the antisense strand. Red arrows: position of cleavage of the chimeric intermediate. A-F: Projected pathways of the chimeric Tier of globin mRNA amplification. A: Conventional genome-originated mRNA; B: Antisense complement of conventional mRNA; C: Folding of the antisense strand into self-priming configuration, 3’terminal “c” is a transcript of the 5’capG of mRNA; D: Extension of self-primed antisense strand into sense-oriented sequence. E: Projected chimeric RNA end product. F: Projected cleavage-generated 3’-truncated antisense RNA. G-I: Selection of detected 5’-truncated sense strand sequences containing 5’-terminal poly(U) segments (highlighted in grey). Note that truncations are clustered around the nucleotide positions corresponding to the projected truncations of the antisense RNA by-product of the chimeric amplification pathways. Top panel: Alpha-globin RNA sequences; bottom panel: Beta-globin RNA sequences.