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. 2019 Oct 25;14(10):e0223084. doi: 10.1371/journal.pone.0223084

Fig 2. Effect of P16-specific methylation on the sensitivity of cancer cells to palbociclib.

Fig 2

(a) Methylation of the CpG P16 island in three cancer cell lines stably transfected with an engineered P16-specific DNA methyltransferase (P16-Dnmt) by MSP. Genomic DNA samples from RKO and MGC803 cells were used as the positive and negative controls for P16 methylation, respectively. (b) Effect of P16-specific methylation on the levels of P16 expression in cell lines stably transfected with P16-Dnmt, as assessed by qRT-PCR. (c) Effect of P16-specific methylation on the viability (survival rate) of cells treated with palbociclib for 48 hrs, as assessed with the IncuCyte ZOOM system. Each point represents the mean ± SD of 4 wells. Student’s t-test, *p<0.05, **p<0.01.