Skip to main content
. 2019 Oct 25;14(10):e0223084. doi: 10.1371/journal.pone.0223084

Fig 3. Selective inhibition of the proliferation of P16-methylated cancer cells by palbociclib in vitro.

Fig 3

After palbociclib treatment (H661 and BGC823 cells: 10 μM; HCC827 cells: 7 μM) for 48 hrs, cells were harvested at 70–80% confluence. (a and b) Effect of palbociclib treatment on the levels of P16-methylated alleles and P16 mRNA in cell lines stably transfected with P16-Dnmt, as assessed by MSP and qRT-PCR assays, respectively. The data are presented as the means ± SDs. (c) The results of immunofluorescence and confocal microscopy analyses to directly detect changes in the subpopulation of cells staining positive for P16 protein within the H661 cell line stably transfected with P16-Dnmt. (d) Illustration of the proliferation of P16 methylated cells selectively inhibited by palbociclib treatment. Scale bar, 400 μm. Student’s t-test, *p<0.05, **p<0.01.