Skip to main content
. 2019 Oct 25;14(10):e0220094. doi: 10.1371/journal.pone.0220094

Fig 1. Assay sensitivity in monitoring AMP, ADP, and ATP concentrations.

Fig 1

(A) Titration of AMP in the presence and absence of 100μM ATP to demonstrate that AMP can be determined in the presence or absence of ATP thus showing no interference from ATP in the reaction since it is removed by adenylate cyclase before conversion of AMP to ATP. The inset is an expanded scale of AMP at lower concentrations to show the sensitivity of the AMP detection. (B) ADP titration, and (C) ATP titration were carried out as mentioned in scheme IA. As shown in the figure, the limit of detection (LOD) reaches 10nM or less. Each point represents an average of a triplicates and results shown are mean ± SD. SD, standard deviation.