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Kinetic parameters for M. extorquens La-, Ce-, and Nd-XoxF, determined using the dye-linked activity assay at 30 °C. Mean ± SD for 3 (La, Ce) or 2 (Nd) independent experiments.
| La-XoxF | Ce-XoxF | Nd-XoxF | |
|---|---|---|---|
| Vmax (nmol min−1 mg−1) | 1860 ± 110 | 1290 ± 100 | 560 ± 30 |
| Km, methanol (μM) | 22 ± 3 | 49 ± 11 | 18 ± 5 |
| kcat (s−1) | 4.0 ± 0.2 | 2.8 ± 0.2 | 1.2 ± 0.1 |
| kcat/Km (μM−1 s−1) | 0.19 ± 0.03 | 0.057 ± 0.014 | 0.067 ± 0.019 |
