REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Fc Block	BD	Cat#553142
CD45 (30-F11)	BD	Cat#564279
EPCAM (G8.8)	Biolegend	Cat#118216
CD31 (MEC13.3)	BD	Cat#565097
CD11b (M1/70; 1:200–1:600)	Biolegend/BD	Cat#101255/Cat#564443
F4/80 (BM8)	eBioscience	Cat#17–4801-82
ICAM2 (3C4)	Biolegend	Cat#105612
PDGFRa (APA5)	Biolegend/BD	Cat#135906/Cat#740269
PDPN (8.1.1; 1:800)	Biolegend	Cat#127410
CD19 (6D5)	Biolegend	Cat#115520
Ly6C (HK1.4; 1:400)	Ebioscience	Cat#53–5932-82
Ly6G (1A8; 1:400)	Biolegend	Cat#127624
MHCII (M5/114.15.2)	Ebioscience	Cat#25–5321-82
SIGLEC F (E50–2440)	BD	Cat#565527
GR1 (RB68C5; 1:400).	Biolegend	Cat#108424
Human HB-EGF (DTR; 1:100)	R&D Systems	Cat#BAF259
Streptavidin BUV396 (1:400)	BD	Cat#564176
GATA6 PE (1:50)	CST	Cat#26452S
Isotype PE (1:100)	CST	Cat#5742S
Fixable Violet Dead Cell Stain Kit	Thermofisher	Cat#L34963
Calcein Blue, AM	Thermofisher	Cat#C1429
Chemicals, Peptides, and Recombinant Proteins
All trans retinoic acid	Tocris	Cat#0695
Retinol	Sigma	Cat#95144
BMS493	Tocris	Cat#3509
MCSF	R&D systems	Cat#416-ML
Dichloromethane	Honeywell Burdick & Jackson	Cat#494453
Methanol	Fisher Chemical	Cat#10675112
RNase Inhibitor	Applied Biosystems	Cat#N8080119
TaqMan Fast Advanced Master Mix	ThermoFisher	Cat#4444557
Diphtheria toxin	EMD Millipore	Cat#322326
Giemsa stain, modified	Sigma	Cat#GS500
Wright stain	Sigma	Cat#WSGD128
RNase Inhibitor	Applied Biosystems	Cat#N8080119
TRIZOL	Invtirogen	Cat#15596026
Collagenase P	Roche	Cat#11249002001
Dispase	Life Technologies	Cat#17105041
DNAse	Roche	Cat#10104159001
Optiprep	Sigma	Cat#D1556
RLT Buffer	QIAGEN	Cat#79216
Critical Commercial Assays
High Capacity cDNA Reverse Transcription Kit	Applied Biosystems	Cat#4368814
RNeasy Plus Mini Kit	Qiagen	Cat#74134
SMARTer Ultra Low Input RNA Kit for Sequencing	Clontech	Cat#634848
Aldefluor Assay	STEMCELL Technologies	Cat#01700
Deposited Data
FACS-sorted fibroblasts from lymph node, spleen, bone, brown adipose, inguinal adipose, omentum, mesentery, pancreas and FACS-sorted mesothelial cells from omentum, mesentery and epididymal adipose		GSE130813
In vitro LCM, Wt1+ stromal cells and ex vivo LCM		GSE130815
Experimental Models: Cell Lines
RARα-luciferase reporter cells	BPS bioscience	Cat#60503
Experimental Models: Organisms/Strains
Mouse: C57BL/6J	Jackson Laboratory	Cat#00064
Mouse: Wt1eGFP.cre	Jackson Laboratory / Genentech	Cat#010911
Mouse: Constitutive GFP+	Jackson Laboratory	Cat#006567
Mouse: IL6−/−	Jackson Laboratory	Cat#002650
Mouse: Lyz2cre;Gata6fl/fl	Gautier et al., 2014	N/A
Mouse: Adiponectincre;Rosa26LSL.DTA	Wu et al., 2018	N/A
Mouse: Wt1eGFP.cre;Rosa26LSL.DTR	This paper	N/A
Mouse: Wt1eGFP.cre;Rosa26LSL.mKATE2	This paper	N/A
Oligonucleotides
Aldh1a1	ThermoFisher	Cat#Mm00657317_m1
Aldh1a2	ThermoFisher	Cat#Mm00501306_m1
Gapdh	ThermoFisher	Cat#Mm99999915_g1
Wt1	ThermoFisher	Cat#Mm01337048_m1
Software and Algorithms
Transcript-level gene expression	Patro et al., 2017	Salmon
Differential expression analysis	Law et al., 2014	limma/voom
FlowJo v9.8.1	FlowJo, LLC	www.flowjo.com
Prism 8.1.0	GraphPad Software	www.graphpad.com
Other
Transwell inserts (0.4µM)	Costar	Cat#3413